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Short‐term effects of CO2 and O2 on citrate metabolism in illuminated leaves

Identifieur interne : 005600 ( Main/Exploration ); précédent : 005599; suivant : 005601

Short‐term effects of CO2 and O2 on citrate metabolism in illuminated leaves

Auteurs : Guillaume Tcherkez [France] ; Aline Mahé ; Florence Guérard ; Edouard R. A. Boex-Fontvieille ; Elisabeth Gout [France] ; Marlène Lamothe ; Margaret M. Barbour [Australie] ; Richard Bligny [France]

Source :

RBID : ISTEX:94DF7EA293E9573C11C85AC79FA4EF0CD3D62362

Descripteurs français

English descriptors

Abstract

Although there is now a considerable literature on the inhibition of leaf respiration (CO2 evolution) by light, little is known about the effect of other environmental conditions on day respiratory metabolism. In particular, CO2 and O2 mole fractions are assumed to cause changes in the tricarboxylic acid pathway (TCAP) but the amplitude and even the direction of such changes are still a matter of debate. Here, we took advantage of isotopic techniques, new simple equations and instant freeze sampling to follow respiratory metabolism in illuminated cocklebur leaves (Xanthium strumarium L.) under different CO2/O2 conditions. Gas exchange coupled to online isotopic analysis showed that CO2 evolved by leaves in the light came from ‘old’ carbon skeletons and there was a slight decrease in 13C natural abundance when [CO2] increased. This suggested the involvement of enzymatic steps fractionating more strongly against 13C and thus increasingly limiting for the metabolic respiratory flux as [CO2] increased. Isotopic labelling with 13C2‐2,4‐citrate lead to 13C‐enriched Glu and 2‐oxoglutarate (2OG), clearly demonstrating poor metabolism of citrate by the TCAP. There was a clear relationship between the ribulose‐1,5‐bisphosphate oxygenation‐to‐carboxylation ratio (vo/vc) and the 13C commitment to 2OG, demonstrating that 2OG and Glu synthesis via the TCAP is positively influenced by photorespiration.

Url:
DOI: 10.1111/j.1365-3040.2012.02550.x


Affiliations:


Links toward previous steps (curation, corpus...)


Le document en format XML

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<term>Ammonium</term>
<term>Ammonium metabolism</term>
<term>Atom position</term>
<term>Atom positions</term>
<term>Australian journal</term>
<term>Average carbon isotope composition</term>
<term>Blackwell publishing</term>
<term>Brassica napus</term>
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<term>Citrate synthase</term>
<term>Citrate synthesis</term>
<term>Citrate uptake</term>
<term>Clear relationship</term>
<term>Compensation point</term>
<term>Cornic</term>
<term>Cousins bloom</term>
<term>Dehydrogenase</term>
<term>Derivative</term>
<term>Different conditions</term>
<term>Different light levels</term>
<term>Different values</term>
<term>Discrimination</term>
<term>Exchange conditions</term>
<term>Experimental botany</term>
<term>Farquhar</term>
<term>Farquhar cernusak</term>
<term>Fractionation</term>
<term>Further details</term>
<term>Gaseous environment</term>
<term>Higher plants</term>
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<term>Isotopic labelling</term>
<term>Isotopic signal</term>
<term>Isotopic techniques</term>
<term>Kirschbaum farquhar</term>
<term>Labelling</term>
<term>Labelling pattern</term>
<term>Laisk</term>
<term>Laisk method</term>
<term>Leaf respiration</term>
<term>Light intensity</term>
<term>Light level</term>
<term>Light levels</term>
<term>Main text</term>
<term>Malate</term>
<term>Metabolic</term>
<term>Metabolism</term>
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<term>Mitochondrial metabolism</term>
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<term>Mole</term>
<term>Mole fraction</term>
<term>Mole fractions</term>
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<term>Nitrogen metabolism</term>
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<term>Pelargonium hortorum</term>
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<term>Ecologie végétale</term>
<term>Feuille végétal</term>
<term>Isotope</term>
<term>Métabolisme</term>
<term>Oxygène Molécule</term>
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<term>Atom positions</term>
<term>Australian journal</term>
<term>Average carbon isotope composition</term>
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<term>Brassica napus</term>
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<term>Carbon dioxide</term>
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<term>Carbon skeletons</term>
<term>Cell environment</term>
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<term>Citrate metabolism</term>
<term>Citrate molecules</term>
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<term>Citrate synthesis</term>
<term>Citrate uptake</term>
<term>Clear relationship</term>
<term>Compensation point</term>
<term>Cornic</term>
<term>Cousins bloom</term>
<term>Dehydrogenase</term>
<term>Derivative</term>
<term>Different conditions</term>
<term>Different light levels</term>
<term>Different values</term>
<term>Exchange conditions</term>
<term>Experimental botany</term>
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<term>Farquhar cernusak</term>
<term>Fractionation</term>
<term>Further details</term>
<term>Gaseous environment</term>
<term>Higher plants</term>
<term>Industrial origin</term>
<term>Isocitrate dehydrogenase</term>
<term>Isotope</term>
<term>Isotope fractionation</term>
<term>Isotope ratio mass spectrometry</term>
<term>Isotopic</term>
<term>Isotopic labelling</term>
<term>Isotopic signal</term>
<term>Isotopic techniques</term>
<term>Kirschbaum farquhar</term>
<term>Labelling</term>
<term>Labelling pattern</term>
<term>Laisk</term>
<term>Laisk method</term>
<term>Leaf respiration</term>
<term>Light intensity</term>
<term>Light level</term>
<term>Light levels</term>
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<term>Metabolic</term>
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<term>Orsay cedex</term>
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<term>Other words</term>
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<term>Paul pellny</term>
<term>Pelargonium hortorum</term>
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<term>Photorespiratory</term>
<term>Photorespiratory conditions</term>
<term>Photorespiratory cycle</term>
<term>Photosynthesis</term>
<term>Photosynthetic</term>
<term>Photosynthetic assimilation</term>
<term>Photosynthetic fractionation</term>
<term>Photosynthetic metabolites</term>
<term>Physiology</term>
<term>Picea sitchensis</term>
<term>Plant physiology</term>
<term>Plant science</term>
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<term>Pyruvate dehydrogenase</term>
<term>Relative humidity</term>
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<term>Respiration rate</term>
<term>Respiratory component</term>
<term>Respiratory metabolism</term>
<term>Respired</term>
<term>Response curve</term>
<term>Right branch</term>
<term>Schmidt kexel</term>
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<term>Tcap</term>
<term>Tcherkez</term>
<term>Ternary effects</term>
<term>Tricarboxylic acid pathway</term>
<term>Water vapour</term>
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<term>étiquetage</term>
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<front>
<div type="abstract" xml:lang="en">Although there is now a considerable literature on the inhibition of leaf respiration (CO2 evolution) by light, little is known about the effect of other environmental conditions on day respiratory metabolism. In particular, CO2 and O2 mole fractions are assumed to cause changes in the tricarboxylic acid pathway (TCAP) but the amplitude and even the direction of such changes are still a matter of debate. Here, we took advantage of isotopic techniques, new simple equations and instant freeze sampling to follow respiratory metabolism in illuminated cocklebur leaves (Xanthium strumarium L.) under different CO2/O2 conditions. Gas exchange coupled to online isotopic analysis showed that CO2 evolved by leaves in the light came from ‘old’ carbon skeletons and there was a slight decrease in 13C natural abundance when [CO2] increased. This suggested the involvement of enzymatic steps fractionating more strongly against 13C and thus increasingly limiting for the metabolic respiratory flux as [CO2] increased. Isotopic labelling with 13C2‐2,4‐citrate lead to 13C‐enriched Glu and 2‐oxoglutarate (2OG), clearly demonstrating poor metabolism of citrate by the TCAP. There was a clear relationship between the ribulose‐1,5‐bisphosphate oxygenation‐to‐carboxylation ratio (vo/vc) and the 13C commitment to 2OG, demonstrating that 2OG and Glu synthesis via the TCAP is positively influenced by photorespiration.</div>
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